Centre de Recerca i Innovació de Catalunya. Spain.Agrupación Empresarial Innovadora del Sector Proveedor de Bienes y Servicios del Sector Oleícola. Spain.Citoliva. Spain.Ethnikh Diepaggelmatikh Organosh Elaidoladoy Kai Elias Astiki Etaireia. Greece.FoodStandard. Greece.Associação dos Agricultores do Ribatejo. Portugal.Sociedade Agrícola Ouro Vegetal. Portugal.Comercial Técnica y Viveros. Spain.Azienda Agricola Pelosi. Italy.Forsite Diagnostics Ltd. United Kingdom.Food and Environment Research Agency. United Kingdom.Wageningen University and Research Centre. Netherlands.Loewe®. Germany.
main goals of the projectscientific objectives
 

Verticillium wilt is occurring with increasing frequency and severity in most olive growing areas of the Mediterranean basin due to intensive farming, use of infected but symptomless plant propagation material or planting in contaminated soil.

The state of the art to measure Verticillium dahliae inoculums levels in soil and in plant currently comprises visual inspection, plating methods, bioassays, immunoassays and molecular methods (in particular PCR or real time PCR). These methods are time consuming and require trained personnel as well as expensive equipment that cannot be deployed in the field.

VERTIGEEN addresses the need for a rapid and reliable onsite detection and quantification device of the Verticillium dahlia fungus in soils and plant samples.  The system will allow associated olive producers to reduce the losses caused by this pathogen and its spread by means of precise and efficient field interventions within an Integrated Pest Management (IPM) strategy.

VERTIGEEN system innovatively integrates, in a single platform, new but successfully demonstrated technologies: the loop mediated isothermal amplification (LAMP) method and the original in-solution-DNA electrochemical detection, overcoming the limitations of the state of the art in terms of heterogeneity, portability, price, ease of use and time-to-result.